RNA Methodologies. Laboratory Guide for Isolation and Characterization PDF

RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise.

- Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more
- Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies
- Presents comprehensive, cutting-edge information covering all aspects of working with RNA
- Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application
- Presents multiple avenues for addressing the same experimental goals

RNA方法论，第五版延续了其优良传统，为经验丰富的科学家和研究生提供最新的核糖核酸实验室技术。本版收录了关于microRNA这个爆炸性领域的新材料，以及基因表达的剖析方法，这两方面近年来都发生了很大的变化。作为该领域的领军人物，Farrell博士提供了丰富的RNA主题知识，同时也从他个人在这一主题领域的经验中给予读者有益的提示。从最现代的RNA方法论开始，《RNA方法论》第五版为有经验的从业者介绍了处理RNA时的基本技术，同时提供了图像和实例，帮助初学者充分了解分子生物学的这个重要分支。下一代科学家可以把这部作品看作是确保高生产率和高代表性数据的指南，以及在出现实验室问题时排除故障的最佳实践。

- 设有miRNA、MIQE指南、生物标志物、RNA测序、数字PCR等方面的新材料。

- 包括定量PCR技术、RNAi、生物信息学、锁定核酸的作用、适配体生物学、PCR阵列等现代技术的扩展报道。

- 提供全面的、最前沿的信息，涵盖RNA工作的所有方面。

- 从RNA技术的基本信息到深入的协议，再到如何修改和调整特定应用的每个步骤的指导。

- 为解决同一实验目标提供了多种途径。